Abstract
M. longifolia is a plant with a seed rich of edible oil (more than 50%), but is still under-utilized for edible purposes in Sri Lankan context. It shows a wide distribution throughout the country representing several agro-climatic zones. No studies have done yet to discover variations of M. longifolia seed oil with respect to its different geographical locations. In this study, the content of β-carotene in M. longifolia seed oil samples obtained from four different agro-climatic zones in Sri Lanka was evaluated. The effect of heat on the stability of β-carotene in M. longifolia seed oil was also studied. Dried, fallen seeds were collected from randomly selected trees in four agro-climatic zones in Sri Lanka named low country dry zone (LD), low country wet zone (LW), low country intermediate zone (LI) and mid country intermediate zone (MI). Oil was extracted with a small scale, mechanical oil expeller (cold pressed method). β-carotene content in four samples was assessed with MPOB test method using Ultraviolet-Visible (UV-VIS) spectrophotometer and with High Performance Liquid Chromatography (HPLC) using Chase et al., (1994) method. A series of heat treatment (50 °C - 300 °C) was given and the content of β-carotene was determined at each temperature with the above mentioned two methods. There were some differences in the content of β-carotene for two types of methods. β-carotene content varies from 17.69 to 13.51 ppm in four agro-climatic zones for HPLC method and 20.46 - 27.69 ppm for spectrophotometric method. The reduction of β-carotene content up to 150 °C from the room temperature (30 °C) was not prominent. But after 150 °C, a sudden, sharp decrease was reported. Nutritional composition of seed cake varied significantly (p <0.05) among the different agro-climatic zones. Protein content, similar to Palm kernel was reported which ranged from 15.44 - 17.76%.
Highlights
M. longifolia is a large grown, woody tree of familySapotaceae and reported to have its origin in Southeast Asia
The objective of this study was to determine the β-carotene content in M. longifolia seed oil grown in different agro-climatic zones in Sri Lanka and to assess the stability of β-carotene prior to any heat treatment and after a series of heat treatments
Only the oil sample obtained from low country dry zone (LD) was heated to 50 °C, 70 °C, 100 °C, 150 °C, 200 °C, 250 °C and 300 °C to determine the stability of β-carotene
Summary
M. longifolia is a large grown, woody tree of familySapotaceae and reported to have its origin in Southeast Asia. The objective of this study was to determine the β-carotene content in M. longifolia seed oil grown in different agro-climatic zones in Sri Lanka and to assess the stability of β-carotene prior to any heat treatment and after a series of heat treatments. The dried, fallen fruit seeds of M. longifolia were collected from four different agro-climatic zones in Sri Lanka during August-December 2014 using the random sampling method.
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