Влияние острого плавательного стресса на поведенческие и нейрохимические эффекты производного пиразоло[C]пиридина ГИЖ-72 и диазепама у мышей BALB/c и C57BL/6

Abstract

We have studied the impact of acute swimming stress on the behavioral and neurochemical effects of the pyrazole[c]pyridine derivative GIZh-72 (20 mg/kg, i.p.) and diazepam (1 mg/kg, i.p.). It was shown that anxiety-like behavior in open field test in BALB/c mice and marble burying test in C57BL/6 mice increased 1 hour after acute swimming stress. Decreasing of anxiety-like behavior in marble burying test was occurred in C57BL/6 mice and in open field test in both BALB/c and C57BL/6 mice 24 hours after acute stress. An increase of serotonin and a decrease of norepinephrine levels were registered in the hypothalamus in BALB/c and C57BL/6 mice, while an increase of norepinephrine, 3,4-dihydroxyphenylacetic acid levels and 3,4-dihydroxyphenylacetic acid/dopamine ratio were occurred in the prefrontal cortex in BALB/c. These changes correlated with increasing of anxiety-like behavior, but reversal of these neurochemical changes after treatment with GIZh-72 or diazepam or 24 hours after the stress was coincident with attenuation of anxiety-like behavior. Treatment with GIZh-72 or diazepam without the stress led to a decrease of anxiety-like behavior in open field test and marble burying test in BALB/c and C57BL/6 mice. The effects of GIZh-72 in open field test and marble burying test persisted, while the effects of diazepam in open field test persisted in C57BL/6 and increased in BALB/c mice 1 hour after the stress. Anxiolytic-like effect of GIZh-72 in open field test increased in BALB/c mice, but attenuated in C57BL/6 mice that accompanied the tendency to sedative effect 24 hours after the stress. Diazepam effects in open field test increased in C57BL/6, but absence in BALB/c mice 24 hours after the stress. The effects of GIZh-72 and diazepam in marble burying test persisted in C57BL/6, but not BALB/c mice 24 hours after the stress.