Abstract
Every efforts to demonstrate the existence of bacteriophage in the lyzate produced by the anaerobic lysis of Bac. subtilis H gave negative results, and instead of these two factors were discovered in the filtered lyzate. One of them is the antibiotic substance (s) which specifically kills certain strain of Bac. subtilis and Bac. mesentericus and is fairly thermostable, while the other is the thermolabile lytic factor (the autolysine). This latter factor lyzes both the living and heat killed-Bac. subtilis H from which it was produced it (Fig. 2), and also the related strains of Bac. subtilis, but does not lyze the other species of gram positive bacteria (Sarcina lutea and Staph. aures (Fig. 3)) and Gram negative bacterium (E. coli) which were tested. The logarithmically growing cell of Bac. subti, H was irradiated with ultraviolet light, and after 1. 5 to 2 hours of incubation induced lysis could be observed (Fig. 4).
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