Abstract
熱休克處理可以增進許多植物之耐寒能力,而其确實之作用機制目前尚不清楚。熱休克因子(Hsf)為熱休克反應之主要轉綠調節因子。為深入評估Hsf是否與熱休克啟動之植物耐寒性有關,我們生產出帶有由組成型號啟動子CaMV35S調控之阿拉伯芥(Arabidopsis thaliana)HsfAlb(AtHsfAlb)及β-glucuronidase (gusA )融合基因之轉殖番茄植株。在沒熱處理下,一些熱休克蛋白基因在AtHsfAlb-gusA轉殖株中的表現量,比在野生型或gusA轉殖植株之表現量為高,表示AtHsfAlb-gusA可啟動在番茄轉殖株內的熱休克反應機制。此外,在無熱休克處理下,可溶性抗壞血酸過氧化酵素(ascorbate peroxidase)在AtHsfAlb-gusA 轉殖株中的活性,比野生型或gusA轉殖植株約高兩倍。在無任何熱馴化的條件下,數個AtHsfAlb-gusAL轉殖株系小苗表現出明顯優于野生型或gusA轉殖植株之耐寒性狀。根據這些結果,吾等認為Hsf在熱休克啟動之耐寒性機制中應扮演了重要的角色,而持續表現此轉綠調節因子或許可以應用于增進作物之耐低溫逆境能力。
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