Abstract

The micromycete strain Aspergillus niger VKPM F-719 (B-3), a citric acid producer, can be used to synthesise hydrolytic enzymes by a deep cultivation method on multicomponent carbohydrate substrates. The purpose of this work was to study the dynamics of invertase activity during the cultivation of the Aspergillus niger B-3 strain on glucose and starch hydrolysates. The object of the study was to investigate the use of the Aspergillus niger B-3 micromycete strain, to synthesise citric acid and invertase. Fermentation was carried out periodically using concentrated media technology. Glucose and starch hydrolysates were used as a carbohydrate substrate, with ammonium nitrate acting as a source of nitrogen. Aspergillus niger B-3 fungus strain has the ability to synthesise enzymes with invertase activity when cultivated on glucose and starch hydrolysates. The data obtained can be applied in further studies to develop a technology for the production of citric acid and invertase in a single biotechnological process.

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