第一部份：以微乳液電動層析法分析大黃中之蒽醌類 (anthraquinones)及雙蒽酮類 (bianthrones) 之研究第二部分：以毛細管區帶電泳法進行番瀉製劑中主要成分番瀉苷A (sennoside A) 與番瀉苷B (sennoside B) 之定性與定量分析之研究

Abstract

Part I: Analysis of anthraquinones and bianthrones in rhubarb by microemulsion electrokinetic chromatography Microemulsion eletrokinetic chromatograpy (MEEKC) is a separation technique which is similar in principle to micellar electrokinetic chromatography (MEKC), but with more parameters to be adjusted so as to obtain better separation results. The separation of nine anthraquinones and bianthrones in the present study was investigated for the key-operating variables such as the concentration and the type of surfactant, the type of oil phase, and the addition of organic solvent. The optimal condition was found to be 0.5% (w/w) di-n-butyl-L-tartrate, 1.2% (w/w) 1-butanol, 0.6% (w/w) SDS, 97.7% (w/w) 10 mM Na2B4O7 (pH 9.2) and ACN 30% (v/v), under which these nine compounds could be baseline separated within 20 minuntes. Owing to the complexity of rhubarb constituents, we used solid- phase extraction (SPE) for sample pretreatment. By utilizing suitable proportion of weak acid, weak base, and organic solvent for sample loading, washing, and eluting steps on an Oasis HLB sorbet, most of interference in the crude extracts of rhubarb could be cleaned off. The partially purified extracts with finely tuned concentrations could be injected into capillary electrophoresis. Thus, seven analytes were accurately quantitated. Part II: Analysis of sennoside A and sennoside B in commercial senna tablets by capillary zone electrophoresis Qualitative and quantitative analyses of sennoside A and sennoside B, the major cathartic constituents, in commercial senna tablets was performed in the present study by capillary zone electrophoresis. During the process of method development, we investigated the influence of possible parameters on resolution, including the type, concentration, and pH value of the buffer solution, the temperature, the applied voltage and the addition of organic solvent. The optimal condition was found to be 100 mM CAPS (pH 10.4) at 25 kV and 30