Abstract
The cluster of alternated α- and β-tubulin genes in the genome of Trypanosoma cruzi was shown to be transcribed into a single RNA molecule which upon processing gives rise to the mature α- and β-tubulin mRNAs. This conclusion was based on: (i) nuclear RNA species with the same molecular mass hybridize to both α- and β-tubulin cDNA probes; (ii) S 1 nuclease assay of the clustered tubulin genes has shown protected DNA fragments of the same size and of greater molecular mass than that corresponding to the mRNAs, hybridizable to both α- and β-tubulin cDNA probes; (iii) β-tubulin hybrid selected RNA is still able to hybridize to α-tubulin probe.
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