一个水稻铁转运基因(<italic>OsFRDL1</italic>)参与缺氧诱导根系铁膜形成的调节过程

Abstract

Iron plaques that form on the surface of roots serve as an important nutrient reservoir and as a natural barrier for rice ( Oryza sativa ) seedlings to adapt to environmental stress. However, the molecular mechanism of iron plaque formation is poorly understood. In this study, the Osfrdl1 mutant and its wild-type (Nipponbare) cultivar were used to explore the role of the OsFRDL1 gene in iron plaque formation on rice roots. The results showed that a low phosphorus concentration ( Osfrdl1 mutant than on those of the wild-type. The results of real-time RT-PCR analyses indicated that the stagnant treatment increased the expression of OsFRDL1. As well, OsFRDL1 :: GUS staining indicated that the GUS reporter gene was mainly expressed in epidermis cells and stele tissues in the root. The expression of the reporter gene in these tissues was markedly higher in seedlings in the stagnant treatment than in those in the aerated treatment. In the stagnant treatment, the peroxidase activity was higher in roots of wild-type plants than in those of the Osfrdl1 mutant. Taken together, these results suggest that the stagnant treatment induced increased expression of OsFRDL1 . This increased the peroxidase activity to a higher level in the wild-type than in the mutant, and promoted greater iron plaque formation on the roots.