Abstract
Antibody promoting factor which flowed out into peripheral circulating blood during several hours in early stage after the intravenous invasion of antigen, in rabbits, was separated and identified with “inosine” by Dr. E. SAKAKIBARA et al.In the present paper, the action of inosine was discussed about the net-production of bovine serum gamma globulin which was synthesized in the basic media, instead of measuring antibody which was formed in it during 2 hours after the start of liver slices incuvation. The net-production was measured by the quantitative precipitating method.(1) Normal bovine liver slices formed 115±24.15ug/gr. w.w. of bovine serum gamma globulin as net-production, but when inosine was added to 2.5×10-4M as a final concentration into the basic media, it was increased about 2.5 times as compared with normal bovine liver slices.(2) Several substances related to inosine, i.e. ATP, ADP, AMP, IMP, GMP, hypoxanthine, adenosine and adenine etc., were compared with the activating effect of inosine on the net-prodution of bovine serum gamma globulin. Adenosine displayed an increasing effect as well as inosine, ATP and ADP much less than adenosine, but on the contrary, hypoxanthine, adenine, AMP, IMP and GMP etc, had a little or no effect on the action.
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