Abstract
Diabetes is a metabolic syndrome characterized by high blood sugar levels or hyperglycemia.Alpha-amylase inhibitors can be used as an antidiabetic that controls postprandial hyperglycemia.Alpha-amylase inhibitors are found in plants as protein and non-protein bioactive content such as in lebuiseed. The purpose of this study was to determine the activity of alpha-amylase inhibition of the fraction ofethanol extract of lebui seed. This research was carried out by extraction of lebui seed and then fractionatedlebui extract to obtain n-hexane fraction (HF), ethyl acetate fraction (EAF), and water fraction (WF). Thefractions then tested to alpha-amylase inhibition activity in vitro by measuring the reducing sugar using3.5-dinitrosalicylic (DNS) reagent. Furthermore, the identification of the bioactive content of each fraction wascarried out using thin layer chromatography. The results of alpha-amylase inhibition activity test showed thatall fractions had the ability to inhibit alpha-amylase. The IC50 value of the n-hexane fraction, ethyl acetatefraction, and water fraction were 173.05 mg/ml, 9.98 mg/ml, and 137.19 mg/ml, respectively. Ethyl acetatefraction has the smallest IC50 value compared to other fractions. Based on the identification of the bioactivecontent, bioactive content that is only found in the ethyl acetate fraction but not in other fractions are tannins.Therefore, the chemical content in lebui seed which acts as an alpha-amylase inhibitor might be tannins.
Highlights
Diabetes mellitus is a metabolic syndrome caused by the inability of the pancreas to produce insulin or the loss of the body's ability to effectively use insulin which causes hyperglycemia that can lead to various macrovascular and microvascular complications [1,2]
Treatment of diabetes mellitus aims to control the blood sugar levels of patients to minimize the risk of macrovascular and microvascular complications. α-amylase inhibitors can inhibit the breakdown of carbohydrates to glucose so that glucose absorption decreases. α-Amylase works to hydrolyze carbohydrates into oligosaccharides and dextrins which will be further hydrolyzed to glucose
The results indicate that the lebui seed ethanolic extract and fraction of lebui seed ethanolic extract have the α-amylase inhibitory activities, but no better than acarbose
Summary
Diabetes mellitus is a metabolic syndrome caused by the inability of the pancreas to produce insulin or the loss of the body's ability to effectively use insulin which causes hyperglycemia that can lead to various macrovascular and microvascular complications [1,2]. Treatment of diabetes mellitus aims to control the blood sugar levels of patients to minimize the risk of macrovascular and microvascular complications. Α-amylase inhibitors can inhibit the breakdown of carbohydrates to glucose so that glucose absorption decreases. J.Food Pharm.Sci 2020, 8(2), 266-272 bloodstream [3]. Inhibition of the α-amylase enzyme can delay and prolong the digestion of carbohydrates so that the rate of absorption of glucose decreases and prevents the postprandial hyperglycemia [4]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
