Abstract
The Rhodococcus rhodochrous M33-2nit strain containing in its chromosome two copies of a gene for A. denitrificans В-9582 NitC1 nitrilase under the control of a promoter region of genes of R. rhodochrous M8 nitrile hydratase has been constructed. The culturing of the strain was optimized and it was shown that using a two-substrate cultivation scheme on glucose and acetate, it was possible to obtain up to 17 g cdw/L of cells with the specific activity of 7 units/mg cdw. A capacity of synthesizing ammonium acrylate from acrylonitrile by the cells of A. denitrificans В-9582 and A. rhodochrous M33-2nit under the conditions simulating the industrial process was compared. It was shown that the cells of R. rhodochrous M33-2nit were able to produce ammonium acrylate at higher rates of acrylonitrile feeding, than the cells of A. denitrificans В-9582. Using the R. rhodochrous M33-2nit cells, high-concentration solutions of ammonium acrylate (450 g/L) were obtained with the conversion rate of 99.5%. Rhodococcus rhodochrous, nitrilase, nitrile hydratase promoter, ammonium acrylate, acrylonitrile, biocatalysis.
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