Abstract
The usual rate of actin polymerization is increased if one starts from action nuclei. We have noticed that, using α-actinin crosslinked actin nuclei, the initial net elongation rate is further enhanced. Also initial net depolymerization rates of α-actinin crosslinked F-actin samples are higher than those of controls. These results should imply that α-actinin increases the filament end concentration of actin samples. The experiments with barbed and blocking substances (cytochalasin D and gelsolin-actin complex) confirmed such an increase.We have shown that: (1) α-actinin does not significantly influence actin polymerization over all; (2) α-actinin inhibits the recovery of the filament size in F-actin samples after sonication; and (3) the influence of α-actinin on actin filament end concentration is counteracted by tropomyosin. Therefore, we suggest that, upon filament shearing, α-actinin crosslinking inhibits the annealing of short actin polymers into longer filaments.
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