泌尿器科領域における Acid Phosphatase Isozyme の研究

Abstract

The physico-chemical properties of acid phosphatase isozymes were investigated in the normal human prostate, prostatic tumor, adrenal gland, kidney, seminal vesicle, semen and dog kidney, using pevikon zone electrophoretic and DEAF-Sephadex A-50 column chromatographic techniques. The existence of acid phosphatase isozymes (API and APII) were demonstrated in normal human prostate and prostatic tumor (adenoma and carcinoma). The two acid phosphatase active fractions were obtained on DEAE-Sephadex column chromatography of prostate extract while another active fraction (APK) was obtained in kidney tissue, showing these are isozymes each other. The characteristics of acid phosphatase isozymes of prostate were as follows: The optimum pH of AP I was pH 4.0, and that of AP II was pH 5.0. The Km values of AP I and AP II were 0.166×10-6M and 0.250×10-6M, respectively.These two enzyme proteins were not resistant against heat treatment, although AP II was more stable than AP I. The inhibitory effects of various compounds, such as EDTA, magnesium chloride, sodium fluoride, formaldehyde solution, tartrate, diethylstilbesterol (Honvan), testosterone propionate (Enarmone) were tested. All those affected both isozymes to the same extent.The characteristics of kidney acid phosphatase were as follows:The optimum pH was pH 4.25 and the Km value was 0.570×10-6M. The isozyme (APK) was found to be more resistant than AP I and AP II against heat treatment. APK was strongly inhibited by formaldehyde solution more than AP I and AP II were, and less inhibited by sodium fluoride and tartrate than AP I and AP II were.The isozyme patterns of the bladder, seminal vesicles and semen were similar to those of the prostate, and those of the adrenal gland and dog kidney were similar to those of the human kidney.