Abstract
Investigations aimed at the creation of а standard reagent for the synthesis of 99mTc-labeled ciprofloxacin (antibiotic of the fluoroquinolone group) have been carried out. For isotope labeling, 99mTc eluate from the technetium-99m generator was added to dry sterile mixtures of ciprofloxacin hydrochloride (CFH) with a reducing agent (tin chloride, SnCl2) in various ratios and the mixturtes were incubated for 20 min at room temperature. The influence of components of the reaction mixture on the amount of the radiochemical impurity in the obtained radiopharmaceuticals was estimated using the method of thin-layer chromatography (TLC). The formation of three 99mTc-containing complexes and a colloid related to the hydrolysis of tin compounds is revealed. The content of tin in each complex has been determined by introducing an 117mSn isotope label. Ways to decrease in the colloid formation by changing pH of the medium and by reducing the concentration of SnCl2 in the reagent have been investigated. The reagent composition has been optimized. It is established that radiopharmaceuticals containing less than 0.175 mg SnCl2 per 5 mg CFH can be used without additional filtration. Tests on experimental animals (rabbits) with model inflammation in soft tissues showed the functional suitability of 99mTc-labeled ciprofloxacin for the diagnostics of infectious-inflammatory processes.
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