Abstract
Determination of Oleandomycin was attempted by the following process. A 0.5-cc portion of 1% hydrochloric acid solution of sulfanilic acid was diazotized with 1cc of 1% aqueous solution of sodium nitrite, for 2 minutes in ice water, the resultant solution was shaken with 1cc. of 0.40 N aqueous solution of sodium hydroxide and 0.5cc. of pyridine, 2cc. of aqueous solution of Oleandomycin phosphate (25-300γ/cc. sample solution) was added, and after warming at 30° for 30 minutes, this was shaken vigorously with 3cc. of ethanol and 1cc. of 0.5% aqueous solution of ascorbic acid. This solution colored pink and showed absorption at 532mμ, and the absorbancy was found to be proportional to the concentration, within a range of 25-300γ/cc. of sample concentration.Comparison of values of determination by the present process and by bioassay, using aqueous solution of Oleandomycin phosphate left to stand in a refigerator, at room temperature, and at 37°, for a long period to effect some degree of decomposition, gave well agreeing results. It was found that the aqueous solution of Oleandomycin, when stored at a low temperature, shows almost no decrease in potency even for a long period of time.
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