Abstract
The specific coloration of 1, 8-dihydroxyanthrone derivatives and p-nitrosodimethyl-aniline in pyridine solution was utilized in separtory detecting several kinds of anthrones, especially their 1, 8-dihydroxy derivatives. It was also found that this procedure can be used as a means in studying the component of plants. The choice of the developing agents and Rf values of various anthrone derivatives are respectively shown in Tables I and II. Distribution of 1, 8-dihydroxyanthrone derivatives in plants were examined by the present method, one-dimensional ascending method of paper chromatography (developer: methanol-saturated cyclohexane; coloration agent: ammonia), and with the simplified method using circular filter papers, and some interesting observations were gained (cf. Table III).
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