Abstract
Separatory estimation of putrefaction amines by paper chromatography was examined with 17 compounds. Approximately successful separatory estimation was effected by the one-dimensional ascending method for 20 hours at 25°, using a mixture of butanol, water, and glacial acetic acid (4:2:1) for development. The revelation indicators used were ninhydrin, Dragendorff, diazo, Sakaguchi, and nitric acid, potassium nitrite reagents. In the aliphatic amine series, a relationship was found to hold between the number of carbon atoms in the alkyl chain and the Rf values.Separatory estimation of amines by paper chromatographic method was carried out with the culture solution of squid homogenate inoculated with Escherichia coli or Achromobacter sp., and incubated at 37° for 48 hours. The amines detected were trimethylamine, butylamine, arcaine, tyramine, and histamine.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
