Abstract
ABSTRACT This study was conducted to develop new markers at the region that was related to QTL affecting intra-muscular fat and backfat thickness on chromosome 6q28 - 6q32 in pigs. Dozens of repeated sequences werefounded using shotgun sequencing of several BAC clones corresponding to that region, of which five newmicrostellite markers that identified polymorphism were discovered. The mean number of alleles at each locusobserved 2.13(KP0290F2), 4.63(KP0248C11), 7.38(KP1231C91), 2.75(KP1231C92) and 6.25(KP1231C93) in 8breeds(Landrace, Korean native pig, Duroc, Yorkshire, Berkshire, Wuzhishan pig, Xiang pig, Min pig). Theaverage estimated heterozygosity values at each locus varied from 0.2100(KP0290F2) to 0.8304(KP1231C91) inall populations. In other hand, the average allele of all loci was within range of 0.4517(Berkshire) and 0.6957(Yorkshire). Of these markers, KP0248C11, KP1231C91 and KP1231C93 were identified to have optimalnumber of alleles, high heterozygosity values and low standard deviation values. Especially, KP1231C91 andKP1231C93 might be considered as a useful marker for genetic mapping and diversity study.(
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