Экспериментальная оценка биологических эффектов лазера с длиной волны 532 нм в контактном непрерывном режиме при воздействии на ткани с различными оптическими и механическими свойствами

Abstract

The study presents the results of the experimental action of laser radiation with a wavelength of 532 nm in continuous contact mode on biological tissues with different optical properties. The width of the ablation and coagulation zone, the degree of vaporization of various types of tissues were evaluated. Good coagulation properties of the laser are established when exposed to pigmented tissues. However, the pronounced color dependence characteristic of a given wavelength requires careful selection of the biological objects that are suitable for optical properties. Objectives: An experimental evaluation of the effects of a laser with a wavelength of 532 nm in a continuous contact mode on tissues with different optical and mechanical properties. Materials and methods. We carried out an experimental study of the effects of a laser with a wavelength of 532 nm in a continuous contact mode on biological tissues with different optical and mechanical properties. The crater width and side coagulation zone were measured using an operating microscope and a glass slide with a scale value of 0.1 mm. The tissue samples were weighed before and after the application of a point impact. The increase in laser power contributes to an increase in the width of the incision and the coagulation zone. A more pronounced adhesion of the tissue to the fiber end was noted when exposed to the liver tissue of cattle, which causes smaller values of the ablation zone width in comparison with the muscle tissue of the chicken and is reflected in high values of the error of the mean values. The greatest weight loss with a 2 seconds point impact at a power of 5 W was determined on the muscle tissue of the chicken. Conclusions. Good coagulation properties of the laser have been established when exposed to pigmented tissues, however, the pronounced color dependence characteristic of a given wavelength requires careful selection of the biological objects suitable for optical properties.