생물시료의 3D Tomogram 정밀도 개선을 위한 Band-pass Filtering 활용

Abstract

Electron tomography (ET) of biological specimens is performed from a series of images obtained over a range of tilt angles in a transmission electron microscope. When using the high voltage electron microscope (HVEM), various noises appear in EM images acquired from thick sections by high voltage electron beam. In order to obtain an adequate result in electron tomograms that allow visualization of rather complex and mega-cellular struc- ture such as brain tissue, it is necessary to remove the noise in each original tilt images of thick section. Using band-pass filtering of original tilt images, the filtered images are obtained and used to assemble a reconstructed tomogram. The qualified 3D tomogram from filtered images results in a considerable reduction of the noises compared to conventional tomogram. In conclusion, this study suggests that band-pass filtering is effective to improve the brightness and intensity of HVEM produced tomograms acquired from micron-thick sections of biological specimens. (류근용, 김미 정, 최기주, 제아름, 김수진, 이철현, 정현석, 박종원, 권희석: 생물 시료의 3D Tomogram 정밀도 개선을 위한 Band-pass Filtering 활용)