ピロールによるコルチコイドの定量(第3報)ヒドロコルチゾン, プレドニゾロンおよびコルチゾンのけい光定量

Abstract

Hydrocortisone (HS), prednisolone (PS), and cortisone (CS) were fluorometrically determined by conversion to 21-aldehyde with cupric acetate and by subsequent reaction with pyrrole. Hydrocortisone acetate (HSA), prednisolone acetate (PSA), and cortisone acetate (CSA) were successfully determined by alkaline hydrolysis prior to fluorescence development. The relationship between fluorescence intensity and the concentrations of HS, PS, CS, HSA, PSA, and CSA, showed linearity in the range of 1.25-12.5 ng/ml for HS, PS, and CS, and 2.5-30 ng/ml for HSA, PSA, and CSA. This method was applied to commercial ointments and eye ointments, and the analytical data were compared with those by BT and INAH methods. The values were almost identical to those by BT and INAH methods.