Abstract
Objective To investigate the effect of celecoxib,a selective COX-2 inhibitor,on the expression of multidrug resistance gene(MDR1) and P-glycoprotein(P-gp) in human tongue cancer drug-resistance cell line Tca8113/BLM.Methods Human tongue squamous cell carcinoma cell line Tca8113 cells were repeatedly treated with BLM(30μg/ml) to establish drug-resistant cell line Tca8113/BLM.The inhibitory effect of celecoxib on Tca8113/BLM cell line was determined by MTT assay.The expression of P-gp and MRP were detected by flow cytometry and RT-PCR,respectively.Results The MTT results suggested that celecoxib inhibited the growth and proliferation of Tca8113/BLM cell lines in dose-dependent manner.Celecoxib treatment also resulted in significant down-regulated expression of MDR1 mRNA and P-gp.Conclusion Celecoxib shows a significant effect on inhibiting expression of MDR1 mRNA and P-gp in Tca8113/BLM cells,this is probably related to enhancing the lethal effects of anticancer agents against carcinoma cells.
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