小麦-中间偃麦草2A/6St代换系014-459的分子细胞遗传学鉴定

Abstract

<p id="C3"><italic>Thinopyrum intermedium </italic>is a valuable resource for wheat genetic improvement, and numerous wheat-<italic>Th. intermedium </italic>chromosome addition and substitution lines and partial amphiploids have been developed. Zhong 4 is a wheat-<italic>Th. intermedium </italic>partial amphidiploid, which can easily hybridize with wheat and is extensively utilized for wheat improvement. The strain 014-459 was a progeny of partial amphidiploid Zhong 4 descendant of wheat and <italic>Th. intermedium </italic>with some special characteristics such as high crude protein and wet gluten content and partial F₁ of common wheat cultivars and strain 014-459 were sterility while a few others were fertility despite reciprocal cross. Molecular cytogenetic identification of strain 014-459 were detected for speculated contained fragment of <italic>Th. intermedium </italic>chromosomes based on its special characters. Genomic composition of strain 014-459 was detected by FISH, GISH, and PLUG (polymerase chain reaction-based landmark unique gene) marker analysis. Combined FISH/GISH analysis confirmed that a pair of wheat 2A chromosomes in strain 014-459 of the hybrid progeny between wheat-<italic>Thinopyrum intermedium</italic> partial amphidiploid Zhong 4 and wheat was replaced by a pair of St chromosomes from <italic>Thinopyrum intermedium</italic>. PLUG marker analysis verified that this pair belonged to the sixth homologous group. The special characters of strain 014-459 might be attributable to the substitution of <italic>Th. intermedium</italic> St chromosome into wheat. The study of molecular cytogenetic identification of strain 014-459 was probably beneficial to quality improvement and utilization of 6St chromosome of <italic>Th. intermedium</italic> in wheat breeding.