Вплив мультипробіотика “Апібакт®” на продукцію інтерферону й активність 2',5'-олігоаденілат-синтетази в лімфоцитах тимусу щурів за умов гіпоацидності, викликаної омепразолом

Abstract

The interferon (IFN) production by rat thymus lymphocytes and the activity of IFN-induced 2′,5′-oligoadenylate synthetase (2′,5′-OA-synthetase) in these cells were stu­died at hypoacidity of gastric juice evoked by omeprazole injection and also at combined omeprazol and multiprobiotic “Apibact®” treatment. The IFN production by thymocytes of rats with hypoacidity of gastric juice was shown to increase by 2.5±1.2 times in comparison to control (intact animals), the 2′,5′-OAS activity decreased by 28±1.34%. Both the IFN production and enzyme activity were increased comparative to unstimulated cells in response to IFN inducers – phytohemagglutinin A (PHA) (20 µg/ml) and cycloferon (50 µg/ml) treatment in vitro. Administration of “Apibact®” in parallel with omeprazole administration, led to amplification of the PHA action on IFN production and 2′,5′-OAS activity in thymocytes. Probably, the IFN synthesis by thymocytes is stimulated under hypoacidity of gastric juice. It may be a result of activation of immune response to opportunistic microflora colonization of stomach. “Apibact®” exhibit immunomodulatory properties, amplifying the ability of thymocytes to synthesize IFN in response to exoge­nous stimulation in rats with hypoacidity of gastric juice. Keywords: hypoacidity, omeprazol, multiprobiotics, “Apibact®”, thymocytes, 2′,5′-oli­goadenylate synthetase, interferon inducers.