Abstract
Research was aimed to study the effect of human placenta tissue storage at –20°C on the features of procured from it aqueous-saline extracts and their certain fractions. The methods of gel-chromatography, optical spectroscopy and spin probe EPR were used in the research. Spin probe EPR demonstrated the exposure of erythrocytes with fraction of human placenta extracts below 5 kDa as not affecting the microviscosity of erythrocyte cytosol. Along with that we observed the modification of temperature dependence of spin probe mobility under exposure with the same fraction of placenta extracts, stored for 6 months at –20°C. This correlated with a decrease in acid resistance of erythrocytes under the effect of this fraction. Probl Cryobiol Cryomed 2014; 24(1):28–37.
Highlights
Research was aimed to study the effect of human placenta tissue storage at –20°C on the features of procured from it aqueous-saline extracts and their certain fractions
Previous investigations of the human placenta extracts (HPE) effect on structural state of donor blood erythrocytes showed that these cells were convenient and adequate model to study HPE biological activity [17]
The HPEs from fresh placenta tissue and the one subjected to different low temperature exposures exhibited different effects on erythrocytes structural indices, and that correlated with the changes in HPE protein and/or nucleotide composition in response to these exposures [16, 18]
Summary
Research was aimed to study the effect of human placenta tissue storage at –20°C on the features of procured from it aqueous-saline extracts and their certain fractions. Along with that we observed the modification of temperature dependence of spin probe mobility under exposure with the same fraction of placenta extracts, stored for 6 months at –20°C. This correlated with a decrease in acid resistance of erythrocytes under the effect of this fraction. Які відбуваються у тканинах і розчинах біомакромолекул у процесі низькотемпературного зберігання, є однією з важливих задач кріобіології.
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