Abstract
Various physical constants were determined of the principle (MG) of the Mainini reaction, isolated from human placenta, as a product of approximately single component according to electrophoretic and ultracentrifugal examinations. From the determination of its mobility rate (Table I and Fig. 2), isoelectric point of MG was found to lie between pH 4.1 and 4.2. Its diffusion constant was D20W=2.53×10-7cm.2/sec. and sedimentation constant, S20W=2.55×10-13(cm./sec.)/(dyne/g.). The molecular weight of MG calculated from these constants is about 100, 000 and about 150, 000 from light scattering analysis. Such a large difference due to the method of determination was assumed to be caused by the high content of sugars. The MG molecule is assumed to take rod form of extremely elongated type, showing friction ratio f/f0=2.68, axial ratio of prolate 29.4-33.3, and oblate 40-55.
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