δ 15N and δ 13C in the Mondego estuary food web: Seasonal variation in producers and consumers

Abstract

Assessments of temporal variation in stable carbon and nitrogen ratios were used to examine seasonal trends of the water column and benthic food webs in the Mondego estuary (Portugal). There was a marked seasonality in weather and water column conditions, including nutrient supply and chlorophyll concentrations. In spite of the pronounced environmental changes, we found little evidence of seasonal variation in δ 13C and δ 15N of producers and consumers in the Mondego estuary, with a few notable exceptions. Nitrogen isotope ratios in macrophytes ( Zostera noltii, Ulva sp., Enteromorpha sp., and Gracilaria sp.), and in two grazers ( Idotea chelipes and Lekanesphaera levii) increased during late summer, with the highest δ 15N values being measured in July, during a period of elevated temperatures and drought, which may have favored high rates of denitrification and heavier δ 15N values. The results suggest that stable-isotope values from macrophytes and selected grazers are useful as tracers of seasonal changes in nitrogen inputs into estuaries, and that those of consumers reflect other factors beyond seasonal variations in N and C sources.