Abstract
The effect of be polyethylene glycol with a molecular weight of 1500 (PEG-1500) and sucrose on the changes in geometric parameters and osmotic fragility of human erythrocytes was studied. At equal mass fractions of two substances, sucrose caused more pronounced changes in the distribution of erythrocytes according to direct light scattering (FSC) in comparison with PEG. Nevertheless, PEG induced a shift in the distribution of erythrocytes according to the sphericity index in the direction of the spherical cell types, and also increased the osmotic fragility of the erythrocytes relative to the intact and incubated in the presence of sucrose cells. Stomatocytosis was the prevailing trend of the shape modification in the PEG presence, while the effect of sucrose led to the dominance of dehydrated flattened cells. Differences in the external manifestations of the erythrocyte response to PEG and sucrose solutions, accompanied by unequal changes in osmotic fragility, indicate the role of structural membrane modifications induced by cryoprotectants for cell stabilization during subsequent freezing. Probl Cryobiol Cryomed 2017; 27(4): 2 96 – 310
Highlights
The effect of the polyethylene glycol with a molecular weight of 1500 (PEG-1500) and sucrose on the changes in geometric parameters and osmotic fragility of human erythrocytes was studied
The study of the modification of geometric parameters of erythrocytes under the influence of cryoprotectants can be of strong interest for understanding the mechanisms of cell stabilization under stressful conditions, since the change in volume and shape of cells affects physical properties of erythrocyte membranes [15]
The introduction of hypertonic solutions of extracellular cryoprotectants into the suspension of erythrocytes causes water to escape from the cells, the process is followed by a redistribution of ions between the external and intracellular media and a change in the cell volume
Summary
The effect of the polyethylene glycol with a molecular weight of 1500 (PEG-1500) and sucrose on the changes in geometric parameters and osmotic fragility of human erythrocytes was studied. Differences in the external manifestations of the erythrocyte response to PEG and sucrose solutions, accompanied by unequal changes in osmotic fragility, indicate the role of structural membrane modifications induced by cryoprotectants in cell stabilization during subsequent freezing. Для защиты эритроцитов человека от влияния неблагоприятных факторов криоконсервирования в большинстве протоколов используют эндоцеллюлярный криопротектор глицерол [21, 27], который легко проникает через плазматическую мембрану. Однако для поддержания осмотической стабильности эритроцитов в физиологических условиях криопротекторный агент необходимо удалять из размороженных клеточных суспензий, что является длительной и трудоемкой процедурой, а также приводит к значительной потере клеток. Поэтому поиск новых криопротекторов для эритроцитов человека все еще остается актуальным
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