δ 13C and δD compositions of n-alkanes from modern angiosperms and conifers: An experimental set up in central Washington State, USA

Abstract

This study investigates stable carbon and hydrogen isotope compositions of leaf wax n-alkanes from 3 angiosperm and 2 conifer species from a semi-desert natural environment. The δ 13C and δD n-C 27 alkane data show a clear distinction between Populus tremuloides/Syringa vulgaris (−31.9 to −32.7‰ and −168 to −186‰, respectively) and Pinus sylvestris/Picea pungens (−28.8 to −30.6‰ and −190 to −212‰) throughout the 2005 growing season. Like the other angiosperm species, Betula pendula was D-enriched (−162 to −178‰) relative to the conifer species. However, its δ 13C values were more similar to those of Pinus sylvestris and Picea pungens in May, but had a ∼4‰ positive shift later in the season. We suggest that the observed isotopic variations derive mainly from lower stomatal conductance for CO 2 and H 2O vapor in conifers relative to angiosperms. Betula pendula experienced severe environmental stress that affected its carbon metabolism for most of the season. Our results suggest that shifts in δD and δ 13C values of sedimentary leaf waxes may result not only from shifts in the paleoclimatic variables but also from temporal shifts in the distribution of angiosperms and conifers as well as from changes in the isotopic signatures of higher plants undergoing metabolic changes.