鶏糞に処理したアルトシッド 10F^[○!R]のイエバエ羽化阻害効果

Abstract

Laboratory and field tests were performed in order to establish the practical usage of a juvenoid, methoprene as an alternative fly control agent at poultry houses. Effectiveness of methoprene was evaluated based on the inhibition of adult emergence of the house fly, Musca domestica L. with a 10% slow release formulation, Altosid 10F^[○!R]. In the laboratory test with 6-day-old larvae of the field strains the median inhibitory concentration of Altosid 10F in larval medium with poultry droppings was 1.32ppm in active ingredient. The persistence of Altosid 10F in the above medium under 25℃ and 16L-8D photoperiod was assayed with 6-day-old larvae on the 14th, 29th, and 50th day after application. It was assumeP that Altosid 10F in the medium persisted as much as 52.6,43.5,and 19.2% activity of original pereparation on the 14th, 29th, and 50th day, respectively. In the field test 50,25,and 12.5ppm of Altosid 10F were sprayed at 2 liters per square meter by an electric power sprayer onto poultry droppings. Among them 50ppm spray demonstrated an effective control for longer suppression of adult emergence. The emergence inhibition of pupae collected from poultry droppings treated with 50ppm of Altosid 10F were 88.1,74.2,and 46.5% on 7,13 and 19th day after treatment, respectively. The susceptibility of two field strains of fly against methoprene and four commercialized organophosphorus insecticides, diazinon, trichlorfon, dichlorbos, and fenitrothion was evaluated and compared with that of the susceptible laboratory strain, Takatsuki strain, by topical application on 6-day-old larvae. Both field strains indicated high resistance more than 47 fold to all tested organophosphorus insecticides showing maximum resistance level as 330 fold to trichlorfon but no appreciable resistance to methoprene was observed. Therefore, we suggest that methoprene does not have a cross-resistace relation with four tested organophosphorus insecticides in these two field strains of house fly.