Abstract
Total alkaloid assay in Rauwolfia serpentina root was modified to use petroleum ether for preliminary extraction, by which the emulsion was prevented and analytical procedure was improved. The total alkaloid content in domestic plant was measured by this modified method using individuals according to age of the plant and to month of the year. The relative content ratio of strong basic alkaloids was roughly estimated by using ultraviolet spectra of total alkaloids. The pattern on the paper chromatogram of the total alkaloid in the domestic plant was similar to that of the foreign origin. The low content of strong basic alkaloid in the domestic plant seemed to be the distinct difference from the plant of the foreign origin.
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