Abstract
The dried flowers of lily of the valley (Convallaria majalis L. var. Keisukei MAKINO or Convallaria Keisukei MIQ.), growing in the Hokkaido area, was extracted with cold methanol, its residual extract was dissolved in ethanol, instead of purification through the lead salt, and acetone-soluble portion was collected. This portion was dissolved in water, shaken with an ethanol-chloroform mixture (1:9), and a portion transiting to this solvent layer was submitted to liquid chromatography through alumina and developed with methanolic chloroform. Convallotoxin was obtained in an yield of about 0.02% against the dried flower.
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