真菌の生化学的免疫化学的研究 (第1報)

Abstract

The MeOH- and Sephadex-fractionation were done for the isolation of immunological active substances in culture media of three species fungi Aspergillus fumigatus, Rhizopus arrhizus and Trichophyton rubrum.Chemical and serological properties of the extracellular antigenic substances produced by fungi were examined, and the following results were obtained.The component sugars of the polysaccharides were identified as follows. Polysaccharide of Asp. fumigatus was composed of galactose, mannose and glucose as similar to Tri. rubrum, but that of Rhizopus arrhizus was shown to possess mannose and galactose.Hexosamine amount was indicated in a range of 0.5-1.5% with Elson-Morgan method.A large quantity of the yields of MeOH-fractionation were gained in concentration of 75-85% MeOH.Each fractions containing glycoprotein were indicated similarity of paper electrophoretic pattern at nearly starting point.At the serological activity of precipitin reaction, Asp. fumigatus and Tri. rubrum have been shown a largest activity when experiments were tested with 25-75% MeOH-precipited fraction. It was different from the fact that culture filtrate of Rhi. arrhizus have been shown scarcely activity of precipitin reaction.