α 1-Adrenoceptors in the rat cerebral cortex: New insights into the characterization of α 1L- and α 1D-adrenoceptors

Abstract

Among the three α 1-adrenoceptor subtypes (α 1A, α 1B and α 1D) a peculiar intracellular localization and poor coupling to membrane signals of cloned α 1D-adrenoceptor have been reported. In addition, the α 1L-adrenoceptor (low affinity for prazosin), a functional phenotype of α 1A, has been described. The purpose of this work was to analyze the expression, cellular localization and coupling to membrane signalling (inositol phosphate accumulation) of α 1-adrenoceptor subtypes in a native tissue, the rat cerebral cortex. mRNA for the three subtypes was quantified by real-time RT-PCR (α 1D> α 1B ≫ α 1A). α 1-Adrenoceptors were also detected by immunoblotting, revealing α 1A- and α 1B-adrenoceptors to be predominantly expressed in the membrane fraction and the α 1D-adrenoceptor to be localized in the cytosolic fraction. Competitive radioligand binding studies revealed the presence of α 1D-adrenoceptor in tissue homogenates, whereas only α 1A- and α 1B-subtypes were detected in membranes. The proportion of α 1A-adrenoceptor increased after treatment with noradrenaline, suggesting differences in agonist-mediated trafficking. Saturation experiments detected high- and low (α 1A/L)-prazosin binding sites, the latter of which disappeared on incubation with GppNHp. The α 1A/L-adrenoceptor was heavily implicated in the inositol phosphate response, while the α 1D-subtype did not play a relevant role. These results suggest that the predominant cytosolic localization of α 1D-adrenoceptor lies behind its poor coupling to membrane signalling such as inositol phosphate pathway. The fact that the α 1L-adrenoceptor detected in radioligand binding studies disappeared in the presence of GppNHp implies that it represents a conformational state of the α 1A-adrenoceptor coupled to G-protein.