実験的高血圧症に於ける副腎皮質機能の研究(第1報) : 副腎皮質の組織学的並びに組織化学的検索を中心にして

Abstract

The relationship of the adrenal cortex to hypertension has been the subject of a great amount of works and speculations experimentaly and clinically. In spite of an enormous maount of data that have accumulated, this relationship is by no means clear at the present time. From such a point of view, the author has attempted experiments to investigate the relationship between hypertension and adrenal cortex. The present study is to describe the histologic and histochemical responses occurring in the adrenal cortex of animals with hypertension which was produced by various kinds of method as follows : 1) section of the buffer nerves, 2) constriction of renal arteries or encapsulation of the kidney, 3) administration of salt, and 4) injection of desoxy-corticosterone acetate. The determination of 17-ketosteroids in urine was also performed in all experimental hypertensive animals.THE HISTOCHEMICAL METHOD OF STUDY ON THE ADRENAL CORTICAL FUNCTIONS The author applied H. W. Deane's method to detect ketosteroids in the adrenal cortex (Sudan III staining, Schiff's reaction, Schultz reaction, birefringence and aceton solubility).EXPERIMENTAL HYPERTENSION DUE TO DENERATION OF PRESSORECEPTORS The author performed the histologic and histochemical study of the adrenal cortex, determination of 17-ketosteroids in urine to investigate the relationship between experimental hypertension due to denervation of pressoreceptors and the drenal cortex. Ten adult dogs 8.0-16.0 kg. weight, were used in this experiment, and hypertension were produced by cutting the both sides of aortic and carotid sinus nerves according to Hering's method. In the course of experiment, the author clinically investigated body weight, urive volume, blood pressure, histamine test, blood sugar and 17-ketosteroids excretion in urine.After the animals were sacrificed, the author performed the histologic and histochemical examinations of the adrenal cortex. The summary and conclusion of this study are as follows : Section of buffer nerves was followed by hypertrophy and hyperplasia of cells and an appearance of small ketosteroid droplets in zona glomerulosa of the adrenal cortex except one case, namely the signs of hyperfunciton of this zone were observed. But marked changes were not observed in zona fasciculata.There was no relationship between funcitons of zona glomerulosa and elevation of blood pressure. Also an intimate relationship was not found between urinary excretion of 17-ketosteroids and elevation of blood pressure.It is suggested that hypertension produced by section of buffer nerves may develop through the intermediate of adrenaline and/or noradrenaline. But from above results, the author could find no evidence that this hypertension is produced by activation of the pitutitary-adrenal system.