Взаимосвязь цитокинового профиля мононуклеаров и эндотелиальных прогениторных клеток, полученных в результате мобилизации гранулоцитарным колониестимулирующим фактором, у пациентов с хронической сердечной недостаточностью

Abstract

Objective: The aim of the study was to evaluate the ability of cytokine peripheral blood mononuclear cells and its correlation with the release of endothelial progenitor cells after mobilization of G-CSF in patients with chronic heart failure. Methods: Mononuclear cells were obtained from peripheral blood of 35 patients with chronic heart failure before and after mobilization procedures G-CSF (granulocyte colony stimulating factor). Spectrum of cytokine production and growth factors mononuclear cells was evaluated by ELISA in spontaneous conditions and upon stimulation of cells with concanavalin A, lipopolysaccharide, G-CSF, erythropoietin. Results: A statistically significant increase in the spontaneous production of IL-18, VEGF, Epo and reducing TNF-α production and G-CSF mononuclear cells after mobilization procedures G-CSF. A statistically significant increase in mononuclear cell production of IL-18, VEGF, and G-CSF in response to mitogenic stimulation (Con A) and decrease in production of IL-8 after mobilization procedures G-CSF. In response to an antigenic stimulus (LPS) mononuclear cells were enriched with endothelial progenitor cells from patients with chronic heart failure responded statistically significant increase in the production of IL-18 and G-CSF, and decreased production - TNF-α, as compared to similar products of cytokines and growth factors prior to the procedure to mobilize G-CSF. Proangiogenic cytokines G-CSF or Epo result in a statistically significant increase in the production TNF-α, IL-10, VEGF, and G-CSF mononuclear cells enriched endothelial progenitor cells in patients with chronic heart failure. Conclusion: Peripheral blood mononuclear cells, endothelial progenitor cells enriched after mobilization of G-CSF, in patients with chronic heart failure produce cytokines and growth factors with proangiogenic effect. Thus, endothelial progenitor cells contribute to the production of cytokines and growth factors such as TNF-α, IL-18, IL-10, Epo, VEGF. Mononuclear cells were obtained after mobilization of G-CSF, can be used to treat chronic heart failure.