Abstract
Serum ultrafiltrate was obtained from hemodialysis patients by using an extra corporeal ultrafiltration method equipped with a hollow fiber dialyser, and electrophoresed on the SDS-polyacrylamide gel having a high resolving power in the low molecular weight region. Among the major bands less than 40KD observed with plasma of hemodialysis patient, the bands of 29K, 28K, 25K, 22K, 16.7K and 8.9K molecular weights were also found in serum ultrafiltrate of the same patient. By an immunological method, the following bands were identified: α1-microglobulin (28K), retinol-binding protein (22K), prealbumin (16.7K) and β2-microglobulin (8.9K). As compared with serum ultrafiltrate obtained from healthy person, 28K, 25K (in some cases), 22K and 8.9K bands showed obvious increase in cases of hemodialysis patients except for one patient who showed an urine volume of 200ml/day and an electrophoretic pattern closed to that of healthy person.
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