제스프리 그린 키위의 주요 영양성분 및 헥산 분획물의 신경세포 보호효과

Abstract

Zespri green kiwi의 무기질은 K, P, Ca 순으로 많았으며, 아미노산은 glutamic acid, aspartic acid, arginine 순으로 많이 함유되어 있었다. 지방산 함량은 a-linolenic acid, oleic acid 순으로 많이 함유되어 있었으며, 유리당은 fructose 함량이 가장 높았다. Vitamin C와 <TEX>${\beta}$</TEX>-carotene 함량은 각각 29.21 mg/100 g, <TEX>$1.35{\mu}g/100g$</TEX>이었다. Total phenolics는 n-hexane 분획물이 11.83 mg GAE/g으로 가장 높았으며, ABTS radical 소거활성 및 지질 과산화 억제활성에서는 농도 의존적으로 in vitro 항산화 활성이 증가하는 경향을 보였다. 또한 oxidative stress에 대한 신경세포 보호효과 역시 농도의존적 경향을 보여주었다. 본 연구 결과를 종합해볼 때, 생리활성 소재로서의 phenolics를 함유한 kiwi fruit (A.deliciosa)는 in vitro 항산화 활성과 함께 신경세포 보호효과를 기초로 한 고부가 가치 건강지향식품 소재로서의 활용가치가 높다고 판단된다. The physiological characteristics of kiwi (Actinidia delicosa) fruit were analyzed, which inclued its nutritional composition, in vitro-antioxidant activities, and neuronal cell protective effects. The most abundant components of mineral, amino acid, and fatty acid were found to be potassium (K), glutamic acid, and a-linolenic acid, respectively. The major free sugars were fructose, glucose, and sucrose. In addition, <TEX>${\beta}$</TEX>-carotene and vitamin C contents were <TEX>$1.35{\mu}g/100mL$</TEX> and 29.21 mg/100 g, respectively. The 2,2'-azinobis-(3-ethylbenothiazline-6-sulfonic acid) (ABTS) radical-scavenging activity of the n-hexane fraction obtained from the kiwi extract was 10.52% at a concentration of <TEX>$1000{\mu}g/mL$</TEX>. The malondialdehyde (MDA) inhibition of the n-hexane fraction was found to increased in a dose-dependent manner. The intracellular reactive oxygen species (ROS) accumulation after hydrogen peroxide (<TEX>$H_2O_2$</TEX>) treatment of PC12 cells was significantly reduced in the presence of the n-hexane fraction compared to PC12 cells treated with <TEX>$H_2O_2$</TEX> only. Moreover, in the a MTT assay, the n-hexane fraction showed in vitro-protective effects against <TEX>$H_2O_2$</TEX>-induced neurotoxicity.