경동맥 혈전 유발 동물모델을 이용한 한약재 추출물의 효능 검색

Abstract

Objectives : The aim of this study was to examine anti-thrombotic effect of traditional herbal extracts in a rat model of ferric chloride (<TEX>$FeCl_3$</TEX>)-induced carotid arterial thrombosis. Methods : Thirty minutes prior to a 35% <TEX>$FeCl_3$</TEX> application, Sprague Dawley(SD) rats were injected with the 10 types of traditional herbal extracts (100mg/kg, intraperitoneal injection), respectively. The effect of these herbal extracts was examined for time to occlusion(TTO) using the Laser doppler flow meter and measured for thrombus weight (TW) in <TEX>$FeCl_3$</TEX>-induced thrombosis model. Results : In the TTO, Salvia miltiorrhiza (Sm, <TEX>$2.30{\pm}0.28$</TEX> min, p<0.001) and Santalum album (Sa, <TEX>$2.19{\pm}0.19$</TEX> min, p<0.001) showed significantly delayed TTO more than twice compared with Saline-treated group. Cnidium officinale (Co), Psoralea corylifolia (Pc), Scutellatia baicalensis (Sba), Panax notoginseng (Pn), Angelica tenuissima (At), Scrophularia buergeriana (Sbu), Rhus verniciflua (Rv) and Picrasma quassioides (Pq), except for Rhus verniciflua (Rv) also meaningfully impeded TTO more than one fold. In addition, Salvia miltiorrhiza, Santalum album, Cnidium officinale, Psoralea corylifolia and Scutellatia baicalensis significantly reduced TW more than 10% compared with Saline-treated group. Especially, Salvia miltiorrhiza and Santalum album showed the most excellent anti-thrombotic effect among the 10 herbal extracts tested on the restoration of altered TTO and TW. Conclusions : These results suggest that Sm and Sa extracts have outstanding anti-thrombotic effect in <TEX>$FeCl_3$</TEX>-induced thrombosis model and is potentially useful as herbal medicines for the treatment and prevention of thrombosis.