Abstract
Ten mouse monoclonal antibodies (mAbs) against human IgG were produced to apply to antiglobulin reagent. Of these 10mAbs, 5 reacted with heavy (H) chains and 4 recognized light (L) chains of human IgG in Western blot analysis. The specificity of a remained mAb, which gave strong positive reaction in enzyme-linked immunosorbent assay using human IgG as antigen, was not defined by Western blot analysis. All mAbs reacted with human IgG sensitized red blood cells on membrane immunofluorescence assay, but anti-H chain mAbs only exhibited strong positivity on antiglobulin tests using human IgG sensitized red blood cells. These results suggest that the anti-H chain mAbs are important as an antiglobulin reagent.
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