輸血によるＢ型肝炎ウイルス（ＨＢＶ）感染例でのウイルスゲノム解析 供血者および患者におけるｑｕａｓｉｓｐｅｃｉｅｓの解析について

Abstract

To investigate the homology of hepatitis B virus (HBV) genomes in a post-transfusion HBV infection case, PCR direct sequencing was performed using 340 nucleotides in the pre-S1 and pre-S2 regions of HBV DNA isolated from a recipient's serum sample and from a donor's stored blood sample in which HBV DNA was detected. Two different nucleotides were determined at nt 3051 (A/G) and nt 3117 (G/T) in the sequences from the donor's blood and recipient's serum samples. For precise analysis, the PCR products were cloned, and the DNA sequence was determined in the clones. Four and six sequences were obtained from the clones from the donor's blood and recipient's serum samples, respectively. These sequences slightly differed at three positions, namely nt 3051 G/A, nt 3070 G/A and nt 3117 G/T. Each sequence determined from the donor's blood sample corresponded to one from the patient's serum sample. However, the most predominant sequences from the donor's blood and patient's serum samples differed at nt 3051 (A/G) and nt 3117 (G/T). It was considered that the difference in the ratio of quasispecies between the donor and recipient resulted in a discrepancy between sequences determined by PCR direct sequencing.