Abstract
In this study, the author has analyzed cell population kinetics in oral squamous cell carcinoma from the distribution patterns of nuclear DNA and RNA contents in the lesion and pursued the relationship of the result with the clinicopathological findings and the effects of radiation and chemotherapeutic agents. The number of subject cases was 48 and the determination of nuclear DNA and RNA contents was made using DNA-RNA cytofluorometry with acridine orange fluorescence stain. The auther found the following:1) DNA-RNA scattergrams (cytograms) made for the initial medical examination were divided into 4 types: type I had many cells with low RNA contents (that is, Go phase); type II had many cells going into cell cycle and had some cell populations belonging to different cell cycles; type i increased RNA content along with an increase in DNA content. The presence of a single cell population was also estimated; type Ilf, cell groups with abnormally high RNA contents in the 2C DNA range were seen in addition to the presence of the distribution seen and described in type III.2) The clinical effect of external irradiation or the administration of Peplomycin was observed mainly in type III followed by type N. The cases with poor effect were seen often in type I. The cases in type II showed variance in the clinical effect.3) In the cytograms made during the treatment, type I showed a relative abundance of cells at the G0 phase even after the treatment. Type III and IV showed an early decrease in the cells at the G0 phase and an increase in the cells with high contents of both DNA and RNA, that is, those at the G2M phase or abortive mitotic cells. On the other hand, the cases with high clinical effects among type II underwent changes similar to those of type III and IV, and the cases with low effects tended to show no change in the cytogram even after the treatment or accumulation of the cells at the G0 phase.Thus, it was found that analysis of nuclear DNA and RNA contents in oral squamous cell carcinoma could clarify presence of the cells at the G0 phase and some cell populations belonging to other cell cycles. In addition, analysis made it possible to determine cell population kinetics and was useful as an objective index for clinical sensitivity and evaluation.
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