コナダニ類の成虫, 若虫, 幼虫期別の脂質について

Abstract

The qualitative and quantitative analyses of the lipids were carried out for following 5 species of grain mites, Carpoglyphus lactis (C. l.), Tyrophagus putrescentiae (T. p.), Aleuroglyphus ovatus (A. o.), Lardoglyphus konoi (L. k.) and Dermatophagoides farinae (D. f.). Each mite sample was separated to adult, nymph and larva by a sieving method, and their lipid solution were collected in a chloroform-methanol mixture (2 : 1). Total lipid contents in adult mites were as follows : T. p. 2.1%, L. k. 2.9%, A. o. 2.4%, C. l. 2.6%, and D. f. 1.6% under the wet condition. Thin layer chromatography revealed the same lipid classes in five grain mites; cholesteryl esters, triglycerides, cholesterol, free fatty acids, and monoglycerides as neutral lipids, and phosphatidyl serine, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, phosphatidic acid and cardiolipin as phospholipids. By the quantitative determination of neutral lipids, it was also revealed that triglyceride constituted more than 50% of neutral lipids in the mites of four species except D. f., in which free fatty acids constituted a high proportion as much as 57% of neutral lipids. Thus, there were significant quantitative differences both in the total lipid contents and the profile of the neutral lipids between D. f. and the other four species.