Гетеротопическая ксенотрансплантация фрагментов аорты новорожденных поросят после гипотермической инкубации в консервирующих растворах

Konstantin I Bohuslavskyi,Natalia O Remnyova,Vladimir I Padalko,Natalia M Alabedalkarim,Olena S Protsenko,Yana V Gvozdyuk,Galina A Bozhok

doi:10.15407/cryo29.01.073

Abstract

Nowadays the porcine vessels and heart valves have been widely applied in transplantology. Due to a high level of Gal-α-1,3-Gal (α-Gal epitope) expression, the porcine bioprostheses are specially treated prior to transplantation either to remove this epitope or to deactivate it. Our previous findings showed that certain media, used for hypothermic storage (HS), reduced the α-Gal epitope expression in aortic tissue in vitro. Here, we studied the HS impact in the histidine-tryptophan-ketoglutarate (HTK), Euro-Collins and Dulbecco’s Modified Eagle Medium (DMEM) solutions on aortic graft integrity in vivo. The analysis of aortic wall histological structure and the evaluation of the immunoproteasome subunits LMP2 and LMP7 expression level demonstrated the aortic fragments, subjected to HS in Euro-Collins solution to day 7 after xenotransplantation to cause a less pronounced immune response as compared to the HTK- and DMEM-incubated xenografts. This may be partially due to the Euro-Collins medium composition. Our findings may be of interest for ela-borating the novel methods for donor material pre-transplantation preparation. Probl Cryobiol Cryomed 2019; 29(1): 073–087

Highlights

Nowadays the porcine vessels and heart valves have been widely applied in transplantology
The animals were divided into four groups: the group 1 comprised the animals with xenotransplantation of fragments after hypothermic storage (HS) in HTK (n = 5); the group 2 consisted of the animals with xenotransplanted fragments after HS in Euro-Collins (n = 5); the fragments post HS in Dulbecco’s Modified Eagle Medium (DMEM) made the group 3 (n = 5); those after α-galactosidase treatment (n = 4) represented the group 4
The donor tissue transplantation under the renal capsule of recipient is widely used in experimental transplantology, since the subcapsular space is not an immunologically privileged site, and it allows grafting a sufficient amount of donor tissue and ensures a good vascularization of the graft [30]

Summary

Introduction

Nowadays the porcine vessels and heart valves have been widely applied in transplantology. The analysis of aortic wall histological structure and the evaluation of the immunoproteasome subunits LMP2 and LMP7 expression level demonstrated the aortic fragments, subjected to HS in Euro-Collins solution to day 7 after xenotransplantation to cause a less pronounced immune response as compared to the HTK- and DMEM-incubated xenografts. This may be partially due to the Euro-Collins medium composition. Следовательно, экспрессия иммунных субъединиц протеасом в сайте трансплантации может быть одним из признаков распознавания и презентации чужеродных антигенов

Methods

Results

Conclusion