温度驯化对红耳滑龟幼龟选择体温、热耐受性和抗氧化酶活性的影响

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 温度驯化对红耳滑龟幼龟选择体温、热耐受性和抗氧化酶活性的影响 DOI: 10.5846/stxb201408101597 作者: 作者单位: 温州大学 生命与环境科学学院,温州大学 生命与环境科学学院,温州大学 生命与环境科学学院,温州大学 生命与环境科学学院,温州大学 生命与环境科学学院,温州大学 作者简介: 通讯作者: 中图分类号: 基金项目: 国家自然科学基金项目(31170376);浙江省大学生科技创新活动计划(2013R424029);温州大学实验室开放项目(12SK56A) Influence of temperature acclimation on selected body temperature, thermal tolerance, and antioxidant enzyme activity of juvenile red-eared slider turtles (Trachemys scripta elegans) Author: Affiliation: College of Life and Environmental Sciences,Wenzhou University,College of Life and Environmental Sciences,Wenzhou University,College of Life and Environmental Sciences,Wenzhou University,College of Life and Environmental Sciences,Wenzhou University,College of Life and Environmental Sciences,Wenzhou University,College of Life and Environmental Sciences,Wenzhou University Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:温度驯化对变温动物的生理和行为表现会产生重要影响,不同物种的驯化反应不尽相同。红耳滑龟(Trachemys scripta elegans)为外来入侵种,在中国许多地区发现了野外种群,对当地生物多样性产生了一定程度的威胁。为了探讨红耳滑龟幼体的热适应表现,在5个恒定温度(16,20,26,32℃和36℃)下驯化幼龟30d后,检验幼龟的选择体温(Tsel),耐受低温(CTMin)、耐受高温(CTMax)和耐受温度范围(TRR),以及骨骼肌、心肌和肝脏的过氧化物岐化酶(SOD)活力、过氧化氢酶(CAT)活力和丙二醛(MDA)含量是否受驯化温度影响。结果显示:驯化温度显著影响幼龟的Tsel、CTMin和CTMax,并随驯化温度的升高而升高,但对TRR无显著影响。驯化温度显著影响骨骼肌、心肌和肝脏的SOD活力。驯化温度显著影响肝脏的MDA含量,而对骨骼肌和心肌的MDA含量无显著影响。驯化温度显著影响心肌和肝脏的CAT活力,对骨骼肌CAT活力无显著影响。以上结果表明:温度驯化会影响红耳滑龟幼体选择体温和热耐受能力,符合越热越好假设;同时会影响组织的抗氧化能力。 Abstract:As an important environmental factor, temperature affects the growth, reproduction, physiology, behavior, and offspring phenotype of reptiles. The influence of thermal acclimation on physiological and behavioral performance has been documented in many ectotherm animals, although the acclimation responses of different species may be not identical. The red-eared slider turtle (Trachemys scripta elegans) is an alien invasive species found in China. Wild populations have been found to be a threat to local biodiversity in many areas. In this paper, critical thermal minima (CTMin) and critical thermal maxima (CTMax) are used to assess the thermal resistance of juvenile red-eared slider turtles. In addition, the selected body temperature (Tsel) is used to determine the species' preferred temperature. Our study aimed to determine whether acclimation temperature would affect the Tsel, CTMin, CTMax, thermal resistance range (TRR), the activity of superoxide dismutase (SOD) and catalase (CAT), and the malondialdehyde (MDA) content of the skeletal muscles, cardiac muscles, and liver. In order to examine their thermal adaptive performance at different temperatures, we selected 90 juvenile turtles weighing between 40.0 and 44.3 g, randomly divided them into 5 groups with similar average weights (P=0.141), and acclimated them to five thermal conditions (16, 20, 26, 32℃ and 36℃) for 30 days. After acclimation, Tsel were measured using a thermometer (UT-325) in thermal gradient groove, while CTMin and CTMax were measured in binder incubators. SOD and CAT activity, as well as the MDA content were measured using commercial kits produced by Jiancheng Biotech Co. Ltd., Nanjing, China, according to the manufacturer's instructions. Then, a one-way ANOVA was used to test the effects of acclimation temperature on Tsel, CTMin, CTMax, TRR, SOD and CAT activity, and the MDA content of different tissues. The results showed that acclimation temperature had significant effects on the Tsel (P < 0.0001), CTMin (P < 0.0001), and CTMax (P < 0.01) of juvenile turtles, but had no significant effect on the TRR (P=0.212). Tsel, CTMin, and CTMax increased with acclimation temperature. Acclimation temperature had a significant impact on SOD activity in the skeletal muscles, cardiac muscles, and liver, as well as on the MDA content of the liver (all P < 0.05). However, it had no effect on the MDA content of the skeletal and cardiac muscles (all P > 0.05). There was higher SOD activity in the skeletal and cardiac muscles at high temperatures (26-36℃) than at low temperatures (16-20℃); meanwhile, in the liver, higher SOD activity and MDA content were observed at medium temperatures (20-32℃). Acclimation temperature had a significant effect on CAT activity in the cardiac muscles and liver (all P < 0.05), but no effect on that in skeletal muscles (P=0.55). In cardiac muscles, CAT activity was significantly lower at 36℃ than at other temperatures, while in the liver CAT activity peaked at 16℃. This indicates that acclimation temperature affects the selected body temperature and thermal tolerance of juvenile red-eared slider turtles, with higher temperatures generally having more favorable effects. The antioxidant activity in the tissues was also affected by differences in acclimation temperature. 参考文献 相似文献 引证文献