Влияние вдыхания паров толуола на интенсивность аудиогенного судорожного припадка у крыс линии Крушинского–Молодкиной

Abstract

Аctuality. The significant proportion of human epilepsy is resistant to anticonvulsant drug therapy, which makes actual the problem of search for new alternative antiepileptic therapies. The goal of this work was the analysis of the effects created by the inhalation of toluene vapor (the strong odorant) on epileptic seizures in rats of Krushinsky-Molodkina strain, which is highly prone to audiogenic epilepsy (AE), the tonic convulsions of the musculature as well as audiogenic myoclonic seizures which develop as the result of serial daily sound exposure. Methods. Rats (n=14) were exposed to 15-s inhalation of toluene vapor with the subsequent (in 20 s) testing of their audiogenic seizure intensity. Results. The toluene action induced a significant reduction in tonic and myoclonic seizure intensity in comparison to the background data of AE sensitivity obtained 1 week prior to the toluene test. In 13 of 14 animals, the seizures ended as the clonic seizures (no tonic seizures developed), while all animals developed tonic seizures in the “background” experiment. All rats developed myoclonic seizures (audiogenic kindling) prior to toluene action while no myoclonic seizures were noted after toluene inhalation in all animals. Conclusion. The results obtained complement the previously published data using WAR strain of rats on the relationship between epilepsy and olfactory system. The anticonvulsive effects of toluene vapor inhalation on the expression of AE signs demonstrate both the importance of genetic models usage in such investigations and the importance of olfactory stimulation as the potential anti-convulsant agent.