Abstract
In this study, the antioxidant activities of fractions (methanol, hexane, methylene chloride, butanol, ethyl acetate and water) of Solanum nigrum L. extract was investigated. The contents of total phenolic compounds of each fractions of methanol, butanol, methylene chloride, ethyl acetate, hexane and water are <TEX>$4.41{\pm}0.23%$</TEX>, <TEX>$5.57{\pm}0.35%$</TEX>, <TEX>$9.89{\pm}0.19%$</TEX>, <TEX>$9.86{\pm}0.19%$</TEX>, <TEX>$1.89{\pm}0.04%$</TEX>, and <TEX>$3.18{\pm}0.06%$</TEX>, respectively. For assay of antioxidant activity, 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging activity, reducing power and nitrite scavenging activity are evaluated. In DPPH radical scavenging activity, the highest effect was obtained from the fraction of ethyl acetate. Reducing power is ordered as ethyl acetate > methylene chloride > methanol. In nitrite scavenging activity, the highest activity was 5.5% (butanol fraction), whereas hexane fraction did not detected. Overall, antioxidant activities are closely related the content of phenolic compound in extracts of S. nigrum L.
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