ЭПИДЕМИОЛОГИЧЕСКАЯ СИТУАЦИЯ ПО ДИРОФИЛЯРИОЗУ НА ТЕРРИТОРИИ ОМСКОЙ ОБЛАСТИ

Abstract

The objects of the study were the maps of the epidemiological survey of persons (26 people) infected with dirofilariae for the period 2013-2020 and living in the Omsk region, as well as dirofilaria helminths removed from the invaded residents (26 helminths). The type of helminths was determined by morphological characteristics, as well as by molecular biological methods. Dirofilaria DNA was detected by PCR with species-specific primers. Determination of the sequences of the obtained amplicons was determined by direct sequencing. The level of sequence homology was determined using GenBank. The material was statistically processed using Microsoft Excel 2010. Dirofilariasis is registered in people of all age groups, however, 73.1% of all cases are in people over 40 years old. Among the infected, women predominated, there were almost 2 times more of them than males. The affected people lived either within the city of Omsk and the adjacent recreational zone, or in the southern districts of the region. In 61.5% of cases, the helminth was localized in the organ of vision. According to the results of parasitological studies, in 80.8% of cases of all removed helminths, immature females were identified, in 19.2% - males. All remote helminths are morphologically assigned to the species D. repens. The species of six dirofilariae was confirmed by PCR with species primers and sequencing of amplification products, which showed almost 100% homology with the European sequences of D. repens published in GenBank. On the territory of the Omsk region, from one to four cases of local infection with dirofilariae are recorded annually. Omsk region can be classified as an area with a low risk of infection with dirofilariae, studies are needed to clarify the prevalence of pathogens of dirofilariasis in the northern regions of the region, as well as in general in Western Siberia to assess the risk of infection of the population, especially taking into account climatic changes. Key words: dirofilariosis, parasitic diseases, nematodes transmitted by mosquitoes