Біохімічні маркери вільнорадикального окиснення та обміну ліпідів у щурів з ожирінням, йододефіцитом та ожирінням у поєднанні з йододефіцитом

Abstract

The purpose of the study was the content of lipid and protein peroxidation products, lipid spectrum parameters and the level of aminotransferases in obese, iodine deficient and obese rats in combination with iodine deficiency. Materials and methods. The study was performed on 45 white nonlinear rats weighing 120-180 g, which were divided into three experimental groups: obese rats (1st experimental group, n = 15), iodine-deficient animals (2nd experimental group), obese animals in combined with iodine deficiency (3rd experimental group, n = 15). The control group consisted of 15 intact rats. The content of products of lipid peroxidation and oxidative modification of proteins was determined in the blood and liver tissue of rats. Blood lipid spectrum was assessed by serum levels of triacylglycerols, total cholesterol, high-density lipoproteins and low-density lipoproteins, followed by calculation of the atherogenic factor. The activity of aspartate aminotransferase and alanine aminotransferase was determined in the blood. Results and discussion. It was found that in the liver tissue of rats and blood of experimental groups the content of lipid hydroperoxides and active products that react with thiobarbituric acid increases, which indicates the activation of lipoperoxidation processes. A variety of changes in protein peroxidation in both blood serum and liver tissue of animals of experimental groups was revealed. Regarding the lipid spectrum, the most pronounced differences in the indicators in relation to the control were found in obese animals in combination with iodine deficiency. In this group of animals, cholesterol was increased by 65% in reference to control, triacylglycerol content increased by 52%, low-density lipoprotein exceeded control by 60%, and high-density lipoprotein decreased by 61% in reference to control. The highest activity of aspartate aminotransferase was found in the group of animals with iodine deficiency, and alanine aminotransferase – in the group of obese animals. Conclusion. In the blood and liver tissue of rats with obesity, iodine deficiency and obesity in combination with iodine deficiency increases the content of products of free radical oxidation. The content of cholesterol, triacylglycerols, low-density lipoproteins increases in the blood, the content of high-density lipoproteins decreases, the activity of aspartate aminotransferase and alanine aminotransferase increases. The most pronounced differences in the indicators in reference to the control were found in obese animals in combination with iodine deficiency