Abstract
Monoclonal antibodies (MAbs) specific to the 22-kDa coat protein (CP) of Broad bean wilt virus 2 (BBWV-2) were produced. The 22-kDa CP of the IA isolate expressed in E. coli was used to immunize mice and three independent hybridoma cell lines were screened. In western blot analyses, the MAbs (MAb3-9B, MAb5-12A and MAb6-2A) reacted to the bacterially expressed 22-kDa CP of the IA isolate and most BBWV-2 isolates, but not to some other BBWV-2 isolates and other plant viruses. In immuno-electron microscopy, MAb3-9B and MAb6-2A reacted with BBWV-2 particles to the 10-5 dilution.
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